Tsuji S, Ichioka S, Sekiya N, Nakatsuka T, et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the Eur.... Date of publication 2005 Mar 1;volume 13(2):209-15.
1. Wound Repair Regen. 2005 Mar-Apr;13(2):209-15.
Analysis of ischemia-reperfusion injury in a microcirculatory model of pressure
ulcers.
Tsuji S(1), Ichioka S, Sekiya N, Nakatsuka T.
Author information:
(1)Department of Plastic and Reconstructive Surgery, Saitama Medical School,
Saitama, Japan.
The aim of this study was to establish a pressure ulcer model that visualizes the
microcirculation, and to examine the participation of ischemia-reperfusion injury
in the pathophysiology of pressure ulcers. An original system composed of a new
skin fold chamber and compression device allowed loading quantitative vertical
stress to the skin. An intravital microscopic technique enabled direct
visualization of the microcirculation in the physiological condition and in
response to pressure application. To estimate the effect of ischemia-reperfusion
injury, animals were divided into two groups: the compression-release group (n =
8), in which the animals received four cycles of compression-release which
consisted of 2 hours of compression followed by 1 hour of pressure release; and
the compression alone group (n = 8) in which the animals underwent continuous
compression for 8 hours. Functional capillary density was quantified before the
compression procedure and on day 1 (35 hours) after the first evaluation. The
cyclic compression-release procedure significantly decreased functional capillary
density as compared to continuous compression, indicating that in our
experimental setting repetition of ischemia-reperfusion cycle more severely
damaged the microcirculation than single prolonged ischemic insult. This finding
supports the significant contribution of ischemia-reperfusion injury to the
pathophysiology of pressure ulcers at the level of dynamic in vivo
microcirculation.
DOI: 10.1111/j.1067-1927.2005.130213.x
PMID: 15828947 [Indexed for MEDLINE]
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