Rudolph R, Vande Berg J, Schneider JA, Fisher JC, Poolman WL, et al.
Plastic and reconstructive surgery. Date of publication 1988 Oct 1;volume 82(4):669-77.
1. Plast Reconstr Surg. 1988 Oct;82(4):669-77.
Slowed growth of cultured fibroblasts from human radiation wounds.
Rudolph R(1), Vande Berg J, Schneider JA, Fisher JC, Poolman WL.
Author information:
(1)San Diego Veterans Administration Medical Center, La Jolla, Calif.
To study radiation effect separate from the microcirculation, fibroblasts were
cultured from four patients with radiation wounds. Cells could be grown from
irradiated tissue near the ulcer and from control normal tissue, but no cells
could be cultured from the ulcers. The ability of radiation-treated fibroblasts
to attach to the substrate and form colonies was less than that of unirradiated
cells. Irradiated skin fibroblasts from the four patients had significantly
longer mean generation times than did control cells. During log-phase growth (1
to 9 days), the population doublings of damaged cells were significantly reduced
compared to colonies from normal cells. These data suggest a permanent intrinsic
radiation effect on fibroblasts or a selective ablation of faster-growing
fibroblast subpopulations that is not dependent on decreased blood supply.
PMID: 3420190 [Indexed for MEDLINE]
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