Edsberg LE, Wyffels JT, Brogan MS, Fries KM, et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the Eur.... Date of publication 2012 May 1;volume 20(3):378-401.
1. Wound Repair Regen. 2012 May-Jun;20(3):378-401. doi:
10.1111/j.1524-475X.2012.00791.x.
Analysis of the proteomic profile of chronic pressure ulcers.
Edsberg LE(1), Wyffels JT, Brogan MS, Fries KM.
Author information:
(1)Natural and Health Sciences Research Center, Center for Wound Healing
Research, Daemen College, Amherst, NY 14226-3592, USA. ledsberg@daemen.edu
Analysis of the proteomic profile of pressure ulcers over time is a critical
step in the identification of biomarkers of healing or nonhealing in pressure
ulcers. The wound fluid from 32 subjects with 42 pressure ulcers was evaluated
over 6 weeks at 15 time points. Samples specific to both the interior and the
periphery of the wound bed were collected. Antibody screening arrays, isobaric
tags for relative and absolute quantitation with mass spectrometry and
multiplexed microarrays were used to characterize wound fluid and results were
correlated with clinical outcome. Twenty-one proteins were found to distinguish
between healed and chronic wounds and 19 proteins were differentially expressed
between the interior and periphery of wounds. Four proteins, pyruvate kinase
isozymes M1/M2, profilin-1, Ig lambda-1 chain C regions, and Ig gamma-1 chain C
region, were present in lower levels for periphery samples when compared to
interior samples and six proteins, keratin, type II cytoskeletal 6A (KRT6A),
keratin, type I cytoskeletal 14, S100 calcium binding proteins A7,
alpha-1-antitrypsin precursor, hemoglobin subunit alpha, and hemoglobin subunit
beta, were present in higher levels in periphery samples when compared with
interior samples. S100 calcium binding protein A6, S100 calcium binding protein
A7, and soluble receptor for advanced glycation end-products had higher levels
in the periphery of chronic wounds vs. the interior in planar arrays. A
significant temporal trend was noted for monokine induced by gamma interferon
(MIG), synonomous with chemokine (C-X-C motif) ligand 9 (CXCL9), which increased
as wounds healed and remained nearly constant for ulcers that were not
approaching closure.
© 2012 by the Wound Healing Society.
DOI: 10.1111/j.1524-475X.2012.00791.x
PMID: 22564231 [Indexed for MEDLINE]
