Baldwin C, Potter M, Clayton E, Irvine L, Dye J, et al.
Annals of plastic surgery. Date of publication 2009 Jan 1;volume 62(1):92-6.
1. Ann Plast Surg. 2009 Jan;62(1):92-6. doi: 10.1097/SAP.0b013e31817762fd.
Topical negative pressure stimulates endothelial migration and proliferation: a
suggested mechanism for improved integration of Integra.
Baldwin C(1), Potter M, Clayton E, Irvine L, Dye J.
Author information:
(1)RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood, UK.
Topical negative pressure is an effective technique to promote wound healing and
the integration of skin graft and synthetic dermal equivalents. We describe an
in vitro model to investigate the effect of negative pressure on angiogenesis, a
pivotal step. Dermal fibroblasts or human microvascular endothelial cells were
cultured on Integra and subjected to intermittent or continuous negative
pressure. At fixed intervals of over 120 hours, the Integra was fixed and
assessed for cell migration (microscopy), cell viability (MTS assay), and cell
proliferation (Ki67 immunostaining). Under control conditions, endothelial cells
formed a monolayer and failed to ingress, whereas fibroblasts migrated
throughout the Integra within 24 hours. Negative pressure switches endothelial
cell to a migratory and proliferative phenotype. Ingress is greatest with
intermittent rather than continuous negative pressure. It has no effect on
dermal fibroblast function. This study identifies an important, potential
pro-angiogenic mechanism by which topical negative pressure promotes wound
healing.
DOI: 10.1097/SAP.0b013e31817762fd
PMID: 19131729 [Indexed for MEDLINE]
