Gündoğdu RH, Temel H, Bozkırlı BO, Ersoy E, Yazgan A, Yıldırım Z, et al.
JPEN. Journal of parenteral and enteral nutrition. Date of publication 2017 Aug 1;volume 41(6):1045-1050.
1. JPEN J Parenter Enteral Nutr. 2017 Aug;41(6):1045-1050. doi:
10.1177/0148607115625221. Epub 2016 Jan 29.
Mixture of Arginine, Glutamine, and β-hydroxy-β-methyl Butyrate Enhances the
Healing of Ischemic Wounds in Rats.
Gündoğdu RH(1), Temel H(2), Bozkırlı BO(3), Ersoy E(4), Yazgan A(5), Yıldırım
Z(6).
Author information:
(1)1 Department of General Surgery, Ankara Atatürk Training and Research
Hospital, Ankara, Turkey.
(2)2 Department of General Surgery, Gemerek State Hospital, Sivas, Turkey.
(3)3 Department of General Surgery, Ankara Training and Research Hospital,
Ankara, Turkey.
(4)4 Department of General Surgery, Güven Hospital, Ankara, Turkey.
(5)5 Department of Pathology, Ankara Atatürk Training and Research Hospital,
Ankara, Turkey.
(6)6 Department of Biochemistry, Etimesgut Public Health Laboratory, Ankara,
Turkey.
BACKGROUND: This study investigated the effects of an amino acid mixture
containing arginine, glutamine, and β-hydroxy-β-methyl butyrate on secondary
healing of ischemic wounds in a rat model (N = 18).
METHODS: After the formation of a bipediculated flap on each rat, 2
full-thickness excisional skin wounds (2 × 2 cm) were created on every flap. The
rats were then randomized into the control and treatment groups. Every rat
received standardized rat food throughout the study. The rats in the treatment
group were administered an extra 200 mg/kg of L-arginine, 200 mg/kg of
L-glutamine, and 40 mg/kg of β-hydroxy-β-methyl butyrate per day. Wound sizes
were measured on days 0, 4, 10, and 14. The rats were sacrificed, and the wounds
were excised for biochemical and histologic examination on the 14th day.
RESULTS: As compared with the control group, the treatment group's wound sizes
were significantly smaller on days 10 and 14 ( P < .001), as was its inflammatory
cell accumulation score ( P = .008). There was no significant difference between
the 2 groups in collagen accumulation ( P = .340), granulation tissue maturation
( P = .161), angiogenesis ( P = .387), or reepithelialization ( P = .190) and no
significant difference between hydroxyproline concentrations in wounds ( P =
.287).
DISCUSSION: This amino acid combination seems to have a positive impact on the
secondary healing of experimental ischemic wounds when introduced as a supplement
to the standard diet, and the reduction in the inflammatory process appears to
play a role in this effect.
DOI: 10.1177/0148607115625221
PMID: 26826258 [Indexed for MEDLINE]
