Lapin GA, Hochman B, Maximino JR, Chadi G, Ferreira LM, et al.
Advances in skin & wound care. Date of publication 2016 Apr 1;volume 29(4):169-77.
1. Adv Skin Wound Care. 2016 Apr;29(4):169-77. doi:
10.1097/01.ASW.0000480096.01724.91.
Effects of Lidocaine, Bupivacaine, and Ropivacaine on Calcitonin Gene-Related
Peptide and Substance P Levels in the Incised Rat Skin.
Lapin GA(1), Hochman B, Maximino JR, Chadi G, Ferreira LM.
Author information:
(1)Guilherme A. F. Lapin, MD, is a Graduate Student in the Division of Plastic
Surgery, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil. Bernardo
Hochman, MD, PhD, is an Affiliate Professor in the Division of Plastic Surgery
and the Coordinator of the Sector of Pathologic Scars in that same division at
UNIFESP. Jessica R. Maximino, PhD, is a Neuroscience Researcher in the
Translational Neurology Unit at the Universidade de São Paulo School of Medicine
(USP), São Paulo, Brazil. Gerson Chadi, MD, PhD, is a Professor in the
Experimental Neurology Department, Head of the Pathophysiology-LIM45, and
Coordinator of the Study Group on Neuroregeneration, all at USP. Lydia M.
Ferreira, MD, PhD, is a Professor in the Division of Plastic Surgery at UNIFESP,
Researcher IB CNPq (Brazilian National Council for Scientific and Technological
Development), Brasília, Federal District, Brazil, and Assistant Coordinator of
MED III CAPES (Coordination for the Improvement of Higher Education Personnel),
Brasília, Federal District, Brazil.
OBJECTIVE: To evaluate the effect of 2% lidocaine, 0.5% bupivacaine, and 0.75%
ropivacaine on the release of substance P (SP) and calcitonin gene-related
peptide (CGRP) in skin wounds.
DESIGN: A primary, experimental, analytical, prospective, self-controlled,
blinded study.
SETTING: The study is set in a university research center.
INTERVENTIONS: Twenty-eight Wistar rats were randomly divided into 4 groups:
lidocaine, bupivacaine, ropivacaine, and the control. After general anesthesia, a
local anesthetic or 0.9% saline (control) was injected subdermally along a 2-cm
line on the dorsal midline of each rat; 30 minutes later, an incision
(nociceptive stimulus) was made along this line. The animals were euthanized, and
skin samples were collected from the center of the incision line and sent for
CGRP and SP quantification.
MAIN OUTCOME MEASURES: Quantification of CGRP and SP by Western blotting.
RESULTS: Substance P levels were similar in the lidocaine and ropivacaine groups
but were significantly lower than those of the control group (P = .002); no
significant difference in SP levels was found between the bupivacaine and control
groups. Procalcitonin gene-related peptide levels were significantly lower in the
experimental groups than those in control subjects (P = .009), with no
significant differences among the experimental groups. No significant differences
in CGRP levels were found among all groups. Lidocaine and ropivacaine inhibited
SP release. All 3 local anesthetics inhibited the release of procalcitonin
gene-related peptide, but not the release of CGRP in rat skin.
CONCLUSIONS: Lidocaine and ropivacaine may inhibit neurogenic inflammation by
biochemical pathways activated by SP, whereas bupivacaine seems to have no
influence on this process.
DOI: 10.1097/01.ASW.0000480096.01724.91
PMID: 26978801 [Indexed for MEDLINE]
