Ma Z, Li Z, Shou K, Jian C, Li P, Niu Y, Qi B, Yu A, et al.
International journal of molecular medicine. Date of publication 2017 Nov 1;volume 40(5):1415-1425.
1. Int J Mol Med. 2017 Nov;40(5):1415-1425. doi: 10.3892/ijmm.2017.3131. Epub 2017
Sep 13.
Negative pressure wound therapy: Regulating blood flow perfusion and microvessel
maturation through microvascular pericytes.
Ma Z(1), Li Z(1), Shou K(1), Jian C(1), Li P(1), Niu Y(1), Qi B(1), Yu A(1).
Author information:
(1)Zhongnan Hospital of Wuhan University, Department of Orthopedics, Wuhan
University, Wuhan, Hubei 430071, P.R. China.
Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound
healing by promoting angiogenesis. However, whether blood flow perfusion is
regulated by microvessel maturation and pericytes following NPWT remains unclear,
as well as the exact association between pericytes and collagen type IV. The aim
of this study was to investigate the relevant association between blood flow
perfusion and microvessel maturation and pericytes following NPWT, and to further
explore the underlying molecular mechanisms. We also aimed to investigate the
association between pericytes and collagen type IV. For this purpose, we created
a rat model of diabetic wounds and microvascular blood flow perfusion was
detected using a laser Doppler blood perfusion imager. The expression levels of
angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2),
α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed
through immunohistochemistry, immunofluorescence, RT-qPCR and western blot
analysis. The results revealed that NPWT promoted the overexpression of
angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased
blood flow perfusion coupled with microvessel maturation in the NPWT group at the
later stages (7-10 days) of wound healing. Our results suggested that NPWT can
preferentially enhance vessel maturation and increase the number of pericytes,
thus regulating blood flow perfusion. On the other hand, pericytes and collagen
type IV had a mutual interaction, promoting microvessel maturation.
DOI: 10.3892/ijmm.2017.3131
PMCID: PMC5627868
PMID: 28901392 [Indexed for MEDLINE]
