de Koning HD, van den Bogaard EH, Bergboer JG, Kamsteeg M, van Vlijmen-Willems IM, Hitomi K, Henry J, Simon M, Takashita N, Ishida-Yamamoto A, Schalkwijk J, Zeeuwen PL, et al.
The British journal of dermatology. Date of publication 2012 Jun 1;volume 166(6):1245-54.
1. Br J Dermatol. 2012 Jun;166(6):1245-54. doi: 10.1111/j.1365-2133.2012.10885.x.
Expression profile of cornified envelope structural proteins and keratinocyte
differentiation-regulating proteins during skin barrier repair.
de Koning HD(1), van den Bogaard EH, Bergboer JG, Kamsteeg M, van Vlijmen-Willems
IM, Hitomi K, Henry J, Simon M, Takashita N, Ishida-Yamamoto A, Schalkwijk J,
Zeeuwen PL.
Author information:
(1)Department of Dermatology, Radboud University Nijmegen Medical Centre, PO Box
9101, 6500 HB Nijmegen, The Netherlands. h.dekoning@derma.umcn.nl
BACKGROUND: Recent studies have emphasized the importance of heritable and
acquired skin barrier abnormalities in common inflammatory diseases such as
psoriasis and atopic dermatitis (AD). To date, no comprehensive studies on the
effect of experimental barrier disruption on cornified envelope protein
expression have been performed.
OBJECTIVES: To analyse the effect of experimental skin barrier disruption on the
expression of cornified envelope structural proteins and keratinocyte
differentiation-regulating proteins.
METHODS: We examined mRNA (day 1, 3 and 7) and protein (day 1, 2, 4 and 9)
expression levels of structural proteins and regulatory molecules after sodium
dodecyl sulphate (SDS) application on normal skin, and tape stripping of
uninvolved epidermis of patients with psoriasis and AD and healthy controls.
RESULTS: Upon tape stripping, several structural molecules were significantly
downregulated (at the mRNA level as well as the protein level), including LCE5A,
LCE2B, FLG, FLG2 and LOR, whereas others were upregulated: IVL, SPRR1, SPRR2,
HRNR and most notably LCE3A. The epidermal crosslinking enzymes TGM1, TGM3 and
TGM5 were all upregulated, whereas proteases involved in the desquamation process
(CTSV, KLK5 and KLK7) were downregulated or unaffected. Most results were similar
in SDS-instigated irritant contact dermatitis. There was no significant
difference in response between normal epidermis and nonlesional skin of patients
with psoriasis and AD.
CONCLUSIONS: Skin barrier disruption induces a temporary barrier repair response
composed of increased expression of several cornification-related proteins, and
decreased expression of some structural and desquamation-related proteins.
© 2012 The Authors. BJD © 2012 British Association of Dermatologists.
DOI: 10.1111/j.1365-2133.2012.10885.x
PMID: 22329734 [Indexed for MEDLINE]
