Jones LM, Dunham D, Rennie MY, Kirman J, Lopez AJ, Keim KC, Little W, Gomez A, Bourke J, Ng H, DaCosta RS, Smith AC, et al.
Future microbiology. Date of publication 2020 Mar 1;volume 15():319-332.
1. Future Microbiol. 2020 Mar;15:319-332. doi: 10.2217/fmb-2019-0279. Epub 2020 Feb
26.
In vitro detection of porphyrin-producing wound bacteria with real-time
fluorescence imaging.
Jones LM(1), Dunham D(1), Rennie MY(1), Kirman J(1), Lopez AJ(2), Keim KC(2),
Little W(2), Gomez A(2), Bourke J(3), Ng H(3), DaCosta RS(1)(4), Smith AC(2).
Author information:
(1)MolecuLight Inc., Toronto, ON M5G 1T6, Canada.
(2)Department of Honors Studies, Texas Tech University, Lubbock, TX 79409, USA.
(3)Department of Microbiology, University Health Network/Sinai Health System,
Toronto, ON, M5G 1X5, Canada.
(4)Princess Margaret Cancer Center, University Health Network, Toronto, ON, M5G
2C1, Canada.
Aim: Fluorescence imaging can visualize polymicrobial populations in chronic and
acute wounds based on porphyrin fluorescence. We investigated the fluorescent
properties of specific wound pathogens and the fluorescence detected from
bacteria in biofilm. Methods: Utilizing Remel Porphyrin Test Agar, 32 bacterial
and four yeast species were examined for red fluorescence under 405 nm violet
light illumination. Polymicrobial biofilms, supplemented with δ-aminolevulinic
acid, were investigated similarly. Results: A total of 28/32 bacteria, 1/4 yeast
species and polymicrobial biofilms produced red fluorescence, in agreement with
their known porphyrin production abilities. Conclusion: These results identify
common wound pathogens capable of producing porphyrin-specific fluorescence and
support clinical observations using fluorescence imaging to detect pathogenic
bacteria in chronic wounds.
DOI: 10.2217/fmb-2019-0279
PMID: 32101035
