Chomiski V, Gragnani A, Bonucci J, Correa SA, Noronha SM, Ferreira LM, et al.
Acta cirurgica brasileira. Date of publication 2016 Aug 1;volume 31(8):505-12.
1. Acta Cir Bras. 2016 Aug;31(8):505-12. doi: 10.1590/S0102-865020160080000002.
Keratinocyte growth factor and the expression of wound-healing-related genes in
primary human keratinocytes from burn patients.
Chomiski V(1), Gragnani A(2), Bonucci J(3), Correa SA(4), Noronha SM(5), Ferreira
LM(6).
Author information:
(1)Fellow MSc degree, Division of Plastic Surgery, Department of Surgery,
Universidade Federal de São Paulo (UNIFESP0, Sao Paulo-SP, Brazil. Acquisition
and interpretation of data, technical procedures, manuscript writing.
(2)PhD, Associate Professor, Division of Plastic Surgery, Department of Surgery,
UNIFESP, Sao Paulo-SP, Brazil. Conception, design, intellectual and scientific
content of the study; analysis and interpretation of data; manuscript writing;
critical revision.
(3)Fellow MSc degree, Division of Plastic Surgery, Department of Surgery,
UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical
procedures.
(4)PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery,
UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical
procedures, acquisition of data, manuscript writing.
(5)PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery,
UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, manuscript
writing.
(6)Head, Full Professor, Division of Plastic Surgery, UNIFESP, Researcher
1A-CNPq, Director Medicine III-CAPES, Sao Paulo-SP, Brazil. Intellectual and
scientific content of the study.
PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on
the expression of wound-healing-related genes in cultured keratinocytes from burn
patients.
METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each
group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated
keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls),
and UKC (untreated keratinocytes from controls). Gene expression analysis using
quantitative polymerase chain reaction (qPCR) array was performed to compare (1)
TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5)
TKB versus TKC, and (6) UKB versus TKC.
RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene;
comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4
had no significant difference in relative gene expression; comparison 5 showed 26
down-regulated and 7 up-regulated genes; and comparison 6 showed 25
down-regulated and 11 up-regulated genes.
CONCLUSION: There was no differential expression of wound-healing-related genes
in cultured primary keratinocytes from burn patients treated with keratinocyte
growth factor.
DOI: 10.1590/S0102-865020160080000002
PMID: 27579877 [Indexed for MEDLINE]
