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Bradic J, Petkovic A, Simonovic N, Radovanovic M, Jeremic J, Zivkovic V, Mitrovic S, Bolevich S, Jakovljevic V, Sretenovic J, Srejovic I, et al.
Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, In.... Date of publication 2019 Jun 1;volume 46(4):483-494.
1. Undersea Hyperb Med. 2019 Jun-Jul-Aug - Third Quarter;46(4):483-494. Preconditioning with hyperbaric oxygen and calcium and potassium channel modulators in the rat heart. Bradic J(1), Petkovic A(1), Simonovic N(2), Radovanovic M(3), Jeremic J(1), Zivkovic V(4), Mitrovic S(5), Bolevich S(6), Jakovljevic V(4)(6), Sretenovic J(4), Srejovic I(4). Author information: (1)University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia. (2)Health Center "Vozdovac," Belgrade, Serbia. (3)Clinical Center Kragujevac, Department of Emergency Medicine, Kragujevac, Serbia. (4)University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia. (5)University of Kragujevac, Faculty of Medical Sciences, Department of Pathology, Kragujevac, Serbia. (6)1st Moscow State Medical University IM Sechenov, Department of Human Pathology, Moscow, Russia. The aim of this study was to establish the effect of combined therapy with hyperbaric oxygen (HBO2) therapy and verapamil, amlodipine or nicorandil on functional recovery and oxidative stress markers after ischemia in the isolated rat heart. The study included 48 rats (Wistar albino, male gender, eight weeks old, body weight 200±50g). All animals were exposed to HBO2 treatment over 14 days. Isolated heart rats were perfused by the Langendorff retrograde method at a constant coronary pressure of 70 cm H2O. After stabilization period the hearts were divided into the following groups: HBO2 group (animals exposed to only HBO2 preconditioning); HBO2 + verapamil; HBO2 + amlodipine; andHBO2 + nicorandil (animals pretreated with HBO2 and appropriate pharmacological agent). Afterward, the hearts in all groups were subjected to 20-minute global ischemia and 30-minute reperfusion. Parameters of heart function were registered, including maximum and minimum rate of pressure development, systolic and diastolic left ventricular pressure, heart rate and coronary flow. Levels of pro-oxidants such as index of lipid peroxidation, measured as thiobarbituric acid-reactive substances, nitrites, levels of superoxide anion radicals and hydrogen peroxide were determined in coronary venous effluent. Changes in cardiac tissue were evaluated by hematoxylin and eosin staining. Obtained results clearly indicate that blockage of calcium channel or the activation of adenosine triphosphate-sensitive potassium (KATP) in combination with HBO2 prevented ischemia/reperfusion-induced cardiac deleterious effects, thus contributing to improvement of functional recovery of the heart. However, future studies are certainly necessary for better understanding the mechanisms through which combination of these two maneuvers of preconditioning triggers cardioprotection. Copyright© Undersea and Hyperbaric Medical Society. PMID: 31509904 Conflict of interest statement: The authors of this paper declare no conflicts of interest exist with this submission.
Appears in following Topics:
Investigational HBOT Indications - Preconditioning for Cardiac Surgery