Bradic J, Petkovic A, Simonovic N, Radovanovic M, Jeremic J, Zivkovic V, Mitrovic S, Bolevich S, Jakovljevic V, Sretenovic J, Srejovic I, et al.
Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, In.... Date of publication 2019 Jun 1;volume 46(4):483-494.
1. Undersea Hyperb Med. 2019 Jun-Jul-Aug - Third Quarter;46(4):483-494.
Preconditioning with hyperbaric oxygen and calcium and potassium channel
modulators in the rat heart.
Bradic J(1), Petkovic A(1), Simonovic N(2), Radovanovic M(3), Jeremic J(1),
Zivkovic V(4), Mitrovic S(5), Bolevich S(6), Jakovljevic V(4)(6), Sretenovic
J(4), Srejovic I(4).
Author information:
(1)University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy,
Kragujevac, Serbia.
(2)Health Center "Vozdovac," Belgrade, Serbia.
(3)Clinical Center Kragujevac, Department of Emergency Medicine, Kragujevac,
Serbia.
(4)University of Kragujevac, Faculty of Medical Sciences, Department of
Physiology, Kragujevac, Serbia.
(5)University of Kragujevac, Faculty of Medical Sciences, Department of
Pathology, Kragujevac, Serbia.
(6)1st Moscow State Medical University IM Sechenov, Department of Human
Pathology, Moscow, Russia.
The aim of this study was to establish the effect of combined therapy with
hyperbaric oxygen (HBO2) therapy and verapamil, amlodipine or nicorandil on
functional recovery and oxidative stress markers after ischemia in the isolated
rat heart. The study included 48 rats (Wistar albino, male gender, eight weeks
old, body weight 200±50g). All animals were exposed to HBO2 treatment over 14
days. Isolated heart rats were perfused by the Langendorff retrograde method at a
constant coronary pressure of 70 cm H2O. After stabilization period the hearts
were divided into the following groups: HBO2 group (animals exposed to only HBO2
preconditioning); HBO2 + verapamil; HBO2 + amlodipine; andHBO2 + nicorandil
(animals pretreated with HBO2 and appropriate pharmacological agent). Afterward,
the hearts in all groups were subjected to 20-minute global ischemia and
30-minute reperfusion. Parameters of heart function were registered, including
maximum and minimum rate of pressure development, systolic and diastolic left
ventricular pressure, heart rate and coronary flow. Levels of pro-oxidants such
as index of lipid peroxidation, measured as thiobarbituric acid-reactive
substances, nitrites, levels of superoxide anion radicals and hydrogen peroxide
were determined in coronary venous effluent. Changes in cardiac tissue were
evaluated by hematoxylin and eosin staining. Obtained results clearly indicate
that blockage of calcium channel or the activation of adenosine
triphosphate-sensitive potassium (KATP) in combination with HBO2 prevented
ischemia/reperfusion-induced cardiac deleterious effects, thus contributing to
improvement of functional recovery of the heart. However, future studies are
certainly necessary for better understanding the mechanisms through which
combination of these two maneuvers of preconditioning triggers cardioprotection.
Copyright© Undersea and Hyperbaric Medical Society.
PMID: 31509904
Conflict of interest statement: The authors of this paper declare no conflicts of
interest exist with this submission.
