Sun Q, Sun Q, Liu Y, Sun X, Tao H, et al.
The Journal of surgical research. Date of publication 2011 Nov 1;volume 171(1):41-6.
1. J Surg Res. 2011 Nov;171(1):41-6. doi: 10.1016/j.jss.2010.01.036. Epub 2010 Feb
16.
Anti-apoptotic effect of hyperbaric oxygen preconditioning on a rat model of
myocardial infarction.
Sun Q(1), Sun Q, Liu Y, Sun X, Tao H.
Author information:
(1)Department of Diving Medicine, Faculty of Naval Medicine, Second Military
Medical University, Shanghai, PR China.
BACKGROUND: In our previous study, it was indicated that hyperbaric oxygen
preconditioning (HOP) could induce a preconditioning against myocardial
infarction and promote neovascularization. In this study, attempts were made to
investigate whether a modified short-term pre-exposure protocol could also induce
cardioprotection, and its potential mechanisms.
MATERIALS AND METHODS: Adult male Sprague Dawley rats were divided into seven
groups; group 1 was sham surgery (SHAM) and group 2 was pre-exposed to normal air
(CTL), and the other groups to HOP 1, 6, 24, 48, and 72 h (H1, H6, H24, H48, H72
groups) before permanent ischemia. The infarct size was measured by
triphenyltetrazolium chloride staining, and left ventricular function parameters
were recorded. The extent of apoptosis was determined by terminal
deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive staining.
Caspase-3 activity, Bcl-2, and Bax expression were also measured.
RESULTS: Compared with CTL group, myocardial infarct size was significantly
decreased as well as cardiac cell apoptosis in area at risk zones (AAR) in H48
group. Meanwhile, the activity of caspase 3 was reduced, the ratio of Bcl-2/Bax
expression was up-regulated, and the heart function parameters, including left
ventricular systolic pressure (LVSP), left ventricular diastolic pressure
(LVEDP), +(dP/dt)(max), and -(dP/dt)(max) were also significantly improved after
preconditioning in H48 group.
CONCLUSION: The results indicate that short-term HOP could induce
cardioprotection and may not last for more than 24 h. HOP prevents myocardium
from permanent ischemia injury by suppression of apoptotic pathways.
Copyright © 2011 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.jss.2010.01.036
PMID: 20421116 [Indexed for MEDLINE]
