Oka M, Berking C, Nesbit M, Satyamoorthy K, Schaider H, Murphy G, Ichihashi M, Sauter E, Herlyn M, et al.
Laboratory investigation; a journal of technical methods and pathology. Date of publication 2000 Apr 1;volume 80(4):595-604.
1. Lab Invest. 2000 Apr;80(4):595-604.
Interleukin-8 overexpression is present in pyoderma gangrenosum ulcers and leads
to ulcer formation in human skin xenografts.
Oka M(1), Berking C, Nesbit M, Satyamoorthy K, Schaider H, Murphy G, Ichihashi M,
Sauter E, Herlyn M.
Author information:
(1)The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
Interleukin-8 (IL-8) is a potent chemotactic polypeptide for neutrophils.
However, the role of this cytokine during inflammation remains unclear. Skin
specimens from patients with pyoderma gangrenosum demonstrated IL-8
overexpression in skin ulcers, which suggests a role for IL-8 in the development
of the disease. We therefore constructed a recombinant adenovirus expressing the
complementary deoxyribonucleic acid encoding human IL-8 (IL-8/Ad5) that induces a
2000-fold increase in IL-8 expression of infected human fibroblasts in vitro.
Human skin engrafted to severe combined immunodeficiency mice and then injected
with the recombinant virus demonstrated erythema, an intense perivascular
infiltration of neutrophils, and extravasation of erythrocytes after 8 hours. By
12 hours after injection, neutrophils had accumulated beneath the epidermis,
which then necrotized, and one or more ulcers that remained for approximately 2
weeks were observed. Clinically and histologically, the ulcers resembled pyoderma
gangrenosum. These clinical and experimental findings suggest an etiologic role
of IL-8 in the pathogenesis of pyoderma gangrenosum.
PMID: 10780675 [Indexed for MEDLINE]
