Godman CA, Chheda KP, Hightower LE, Perdrizet G, Shin DG, Giardina C, et al.
Cell stress & chaperones. Date of publication 2010 Jul 1;volume 15(4):431-42.
1. Cell Stress Chaperones. 2010 Jul;15(4):431-42. doi: 10.1007/s12192-009-0159-0.
Epub 2009 Dec 1.
Hyperbaric oxygen induces a cytoprotective and angiogenic response in human
microvascular endothelial cells.
Godman CA(1), Chheda KP, Hightower LE, Perdrizet G, Shin DG, Giardina C.
Author information:
(1)Department of Molecular and Cell Biology, University of Connecticut, Storrs,
CT, USA. Cassandra.Godman@uconn.edu
A genome-wide microarray analysis of gene expression was carried out on human
microvascular endothelial cells (HMEC-1) exposed to hyperbaric oxygen treatment
(HBOT) under conditions that approximated clinical settings. Highly up-regulated
genes included immediate early transcription factors (FOS, FOSB, and JUNB) and
metallothioneins. Six molecular chaperones were also up-regulated immediately
following HBOT, and all of these have been implicated in protein damage control.
Pathway analysis programs identified the Nrf-2-mediated oxidative stress response
as one of the primary responders to HBOT. Several of the microarray changes in
the Nrf2 pathway and a molecular chaperone were validated using quantitative PCR.
For all of the genes tested (Nrf2, HMOX1, HSPA1A, M1A, ACTC1, and FOS), HBOT
elicited large responses, whereas changes were minimal following treatment with
100% O(2) in the absence of elevated pressure. The increased expression of
immediate early and cytoprotective genes corresponded with an HBOT-induced
increase in cell proliferation and oxidative stress resistance. In addition, HBOT
treatment enhanced endothelial tube formation on Matrigel plates, with
particularly dramatic effects observed following two daily HBO treatments.
Understanding how HBOT influences gene expression changes in endothelial cells
may be beneficial for improving current HBOT-based wound-healing protocols. These
data also point to other potential HBOT applications where stimulating protection
and repair of the endothelium would be beneficial, such as patient
preconditioning prior to major surgery.
DOI: 10.1007/s12192-009-0159-0
PMCID: PMC3082642
PMID: 19949909 [Indexed for MEDLINE]
