Gwak SJ, Kim SS, Sung K, Han J, Choi CY, Kim BS, et al.
Cell transplantation. Date of publication 2005 Jan 1;volume 14(10):809-17.
1. Cell Transplant. 2005;14(10):809-17.
Synergistic effect of keratinocyte transplantation and epidermal growth factor
delivery on epidermal regeneration.
Gwak SJ(1), Kim SS, Sung K, Han J, Choi CY, Kim BS.
Author information:
(1)Department of Chemical Engineering, Hanyang University, Seoul, Korea.
Both keratinocyte transplantation and epidermal growth factor (EGF) delivery
stimulate epidermal regeneration. In this study, we hypothesized that the
combined therapy of keratinocyte transplantation and EGF delivery accelerates
epidermal regeneration compared to the single therapy of either keratinocyte
transplantation or EGF delivery. To test this hypothesis, we utilized fibrin
matrix as a keratinocyte/EGF delivery vehicle for epidermal regeneration.
Full-thickness wounds were created on the dorsum of athymic mice, and human
keratinocytes and EGF in fibrin matrix were sprayed onto the wounds to regenerate
epidermal layers (group 1). As controls, human keratinocytes in fibrin matrix
(group 2), EGF in fibrin matrix (group 3), or fibrin matrix alone (group 4) was
sprayed onto the wounds. Spraying keratinocytes suspended in fibrin matrix did
not affect the keratinocyte viability, as the cell viabilities before and after
spraying were not different. EGF was released from fibrin matrix for 3 days. The
wounds were analyzed with histology and immunohistochemistry at 1 and 3 weeks
after treatments. Compared with the control groups, initial wound closure rate
was highest in group 1. Histological analyses indicated that group 1 exhibited
faster and better epidermal regeneration than the other groups.
Immunohistochemical analyses showed that regenerated epithelium in groups 1 and 2
stained positively for human involucrin at 3 weeks, whereas the tissue sections
of the groups 3 and 4 stained negatively. Human laminin was detected at the
dermal-epidermal junction of the regenerated tissues in groups 1 and 2 at 3 weeks
and was not detected in groups 3 and 4. The epidermal thickness of the
regenerated tissues in group 1 was significantly thicker than that of the other
groups at all time points. These results suggest that the combined therapy of
keratinocyte transplantation and EGF delivery is more efficacious for epidermal
regeneration than each separate therapy alone.
PMID: 16454355 [Indexed for MEDLINE]
