Sultan SM, Stern CS, Allen RJ Jr, Thanik VD, Chang CC, Nguyen PD, Canizares O, Szpalski C, Saadeh PB, Warren SM, Coleman SR, Hazen A, et al.
Plastic and reconstructive surgery. Date of publication 2011 Aug 1;volume 128(2):363-72.
1. Plast Reconstr Surg. 2011 Aug;128(2):363-72. doi: 10.1097/PRS.0b013e31821e6e90.
Human fat grafting alleviates radiation skin damage in a murine model.
Sultan SM(1), Stern CS, Allen RJ Jr, Thanik VD, Chang CC, Nguyen PD, Canizares O,
Szpalski C, Saadeh PB, Warren SM, Coleman SR, Hazen A.
Author information:
(1)Institute of Reconstructive Plastic Surgery Laboratories, New York University
Medical Center, New York, NY 10016, USA.
BACKGROUND: Autogenous fat grafting has been observed to alleviate the sequelae
of chronic radiodermatitis. To date, no study has replicated this finding in an
animal model.
METHODS: The dorsa of adult wild-type FVB mice were shaved and depilated. The
dorsal skin was then distracted away from the body and irradiated (45 Gy). Four
weeks after irradiation, 1.5-cc fat or sham grafts were placed in the dorsal
subcutaneous space. Gross results were analyzed photometrically. The animals were
euthanized at 4 and 8 weeks after fat or sham grafting and their dorsal skin was
processed for histologic analysis.
RESULTS: Hyperpigmentation and ulceration were grossly improved in fat-grafted
mice compared with sham-grafted controls. This improvement manifested
histologically in a number of ways. For example, epidermal thickness measurements
demonstrated decreased thickness in fat-grafted animals at both time points (20.6
± 1.5 μm versus 55.2 ± 5.6 μm, p = 0.004; 17.6 ± 1.1 μm versus 36.3 ± 6.1 μm, p =
0.039). Picrosirius red staining demonstrated a diminished scar index in
fat-treated animals at both time points as well (0.54 ± 0.05 versus 0.74 ± 0.07,
p = 0.034; and 0.55 ± 0.06 versus 0.93 ± 0.07, p = 0.001).
CONCLUSION: Fat grafting attenuates inflammation in acute radiodermatitis and
slows the progression of fibrosis in chronic radiodermatitis.
DOI: 10.1097/PRS.0b013e31821e6e90
PMID: 21502909 [Indexed for MEDLINE]
