Yan G, Su Y, Ai G, et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journ.... Date of publication 2004 Nov 1;volume 18(6):497-501.
1. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 Nov;18(6):497-501.
[Study on human amniotic membrane loaded with marrow mesenchymal stem cells and
epidermis cells in promoting healing of wound combined with radiation injury].
[Article in Chinese]
Yan G(1), Su Y, Ai G.
Author information:
(1)The State Key Laboratory of Trauma, Burn and Combined Injury, Institute of
Combined Injury, Third Military Medical University, Chongqing, 400038, PR China.
Heguoy2001@yahoo.com.cn
OBJECTIVE: To investigate the results of human amniotic membrane (HAM) which are
loaded with marrow mesenchymal stem cells (MSCs) and epidermis cells in treating
full-thickness skin defect combined with radiation injury.
METHODS: Eight minipigs were used in this study. Three round full-thickness
wounds (phi3.67cm), which combined with radiation injury, were created on the
dorsum of each side close to the vertebral column in each animal. Among 48
wounds, 24 left side wounds were treated with HAM loaded with MSCs and epidermis
cells as experimental group (group A), 16 right side wounds with simple HAM (HAM
group, group B) and 8 right side wounds with oil gauze as control (group C). The
granulation tissue, reepithelization and wound area were observed after 1,2 and 3
weeks. Immunohistochemistry was performed using vWF as a marker for blood
vessels. Image analysis was employed to test new area of wound at different
interval time and healing rate of wound.
RESULTS: The healing time of group A was 6 to 7 days faster than that of group C
and 5 to 6 days fister than that of group B. After 15-17 days of graft, there
were significant differences in new area of wound and healing rate between group
A and groups B, C (P < 0. 01). New epidermis fully covered whole wound surface in
group A, and their granulation tissue, which contained a lot of vWF, fibroblasts,
capillaries and collagen, grew well. Many inflammatory cells still were seen in
groups B and C, and their contents of vWF, fibroblasts, capillaries and collagen
in granulation tissue were smaller than that in group A.
CONCLUSION: The graft of HAM loaded with MSCs and epidermis cells played an
effective role in promoting healing of wound combined radiation injury with high
quality.
PMID: 15586713 [Indexed for MEDLINE]
