Mohd Hilmi AB, Hassan A, Halim AS, et al.
Advances in wound care. Date of publication 2015 May 1;volume 4(5):312-320.
1. Adv Wound Care (New Rochelle). 2015 May 1;4(5):312-320.
A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded
Healing Model on Rat.
Mohd Hilmi AB(1), Hassan A(2), Halim AS(3).
Author information:
(1)School of Diagnostic and Biomedicine, Faculty of Health Sciences, Universiti
Sultan Zainal Abidin , Kuala Nerus, Malaysia .
(2)Unit of Anatomy, Faculty of Medicine, Universiti Sultan Zainal Abidin , Kuala
Terengganu, Malaysia .
(3)Reconstructive Sciences Unit, School of Medical Sciences, Universiti Sains
Malaysia , Kubang Kerian, Malaysia .
Objective: An engineered skin substitute is produced to accelerate wound healing
by increasing the mechanical strength of the skin wound via high production of
collagen bundles. During the remodeling stage of wound healing, collagen
deposition is the most important event. The collagen deposition process may be
altered by nutritional deficiency, diabetes mellitus, microbial infection, or
radiation exposure, leading to impaired healing. This study describes the
fabrication of an engineered bilayer skin substitute and evaluates its
effectiveness for the production of collagen bundles in an impaired healing
model. Approach: Rats were exposed to 10 Gy of radiation. Two months
postirradiation, the wounds were excised and treated with one of three skin
replacement products: bilayer engineered skin substitutes, chitosan skin
templates, or duoderm©. The collagen deposition was analyzed by hematoxylin and
eosin staining. Results: On day 21 postwound, the irradiated wounds displayed
increased collagen bundle deposition after treatment using bilayer engineered
skin substitutes (3.4±0.25) and chitosan skin templates (3.2±0.58) compared with
duoderm (2.0±0.63). Innovation: We provide the first report on the fabrication of
bilayer engineered skin substitutes using high density human dermal fibroblasts
cocultured with HFSCs on chitosan skin templates. Conclusion: The high density of
fibroblasts significantly increases the penetration of cells into chitosan skin
templates, contributing to the fabrication of bilayer engineered skin substitute.
DOI: 10.1089/wound.2014.0551
PMCID: PMC4432966
PMID: 26005597
